Journal: Molecular Therapy Oncolytics

Article Title: Oncolytic Herpes Simplex Virus Inhibits Pediatric Brain Tumor Migration and Invasion

doi: 10.1016/j.omto.2017.04.002

Figure Lengend Snippet: Oncolytic HSV1716 Reduced Tumor Infiltration in a Mouse Orthotopic Xenograft Model of DIPG (A–G) 5 × 10 5 disaggregated DIPG cells premixed with PBS (A–D) or HSV1716 at 10 PFU/cell in PBS (E–G) were stereotactically injected into the brains of 4- to 5-week-old NOD SCID mice. Tumors were allowed to establish and mice were euthanized when they showed signs of disease (i.e., severe ataxia or > 15% weight loss). Brains were harvested into 4% PFA, sliced into coronal sections, embedded in paraffin, and sectioned to 4-μm thickness. Tissue sections were stained with H&E. Images were taken using the SPOT Insight camera and Leitz DMRB microscope. Images from mouse brain obtained on day 62 post-surgery (A–D), day 123 post-surgery (E and F), and day 78 post-surgery (G). (A) Brainstem section at ×2.5 magnification. (B) Section through the cerebellum at ×10 magnification. (C) Brainstem section of pons at ×20 magnification. (D) Section showing arachnoid and pia at ×20 magnification. Black arrows show representative examples of diffusely infiltrating atypical glial cells. (E) Cross-section of pons and cerebellum at ×2.5 magnification. Red arrow indicates a well-contained section of abnormal-looking cells. (F) ×40 magnification of the boxed area in (E). (G) Brainstem section at ×20 magnification. Black arrows show representative examples of diffusely infiltrating atypical glial cells. C, cerebellum; P, pons.

Article Snippet: Images were taken using the SPOT Insight camera and Leitz DMRB microscope.

Techniques: Injection, Staining, Microscopy